Sequencing:Article Title: Modified helicases
Article Snippet: .. Number of D/E Sequence vs. K/R Dda Homologue Identity to amino # (SEQ ID NO:) Habitat Uniprot Length 1993/% acids C Rma- Rhodothermus Mild D0MKQ2 678 21 −84/+85 2 DSM marinus halophile, (SEQ ID moderate NO: 9) thermophile >65 ° C. Csp Cyanothece sp. Marine B1X365 496 24 −76/+76 5 (SEQ ID (strain ATCC bacterium NO: 10) 51142) Sru Salinibacter Extremely Q25429 421 26 −78/+54 3 (SEQ ID ruber halophilic, NO: 11) 35-45 ° C. Sgo Sulfurimonas Habitat: B6BJ43 500 27 −72/+64 2 (SEQ ID gotlandica GD1 hydrotherma NO: 12) 1 vents, coastal sediments Vph12B8 Vibrio phage Host found M4MBC3 450 27 −62/+47 6 (SEQ ID henriette 12B8 in saltwater, NO: 13) stomach bug Vph Vibrio phage Host found I6XGX8 421 39 −55/+45 5 (SEQ ID phi-pp2 in saltwater, NO: 14) stomach bug Aph65 Aeromonas Host found E5DRP6 434 40 −57/+48 4 (SEQ ID phage 65 in NO: 15) fresh/brackish water, stomach bug AphCC2 Aeromonas Host found I6XH64 420 41 −53/+44 4 (SEQ ID phage CC2 in NO: 16) fresh/brackish water, stomach bug Cph Cronobacter Host K4FBD0 443 42 −59/+57 4 (SEQ ID phage vB CsaM member of NO: 17) GAP161 enterobacteriaceae Kph Klebsiella Host D5JF67 442 44 −59/+58 5 (SEQ ID phage KP15 member of NO: 18) enterobacteriaceae SphlME13 Stenotrophomonas Host found J7HXT5 438 51 −58/+59 7 (SEQ ID phage in soil NO: 19) IME13 AphAc42 Acinetobacter Host found E5EYE6 442 59 −53/+49 9 (SEQ ID phage Ac42 in soil NO: 20) SphSP18 Shigella phage Host E3SFA5 442 59 −55/+55 9 (SEQ ID SP18 member of NO: 21) enterobacteriaceae Yph Yersinia phage Host I7J3V8 439 64 −52/+52 7 (SEQ ID phiR1-RT member of NO: 22) enterobacteriaceae SphS16 Salmonella Host M1EA88 441 72 −56/+55 5 (SEQ ID phage S16 member of NO: 23) enterobacteriaceae 1993 Enterobateria Host P32270 439 100 −57/+58 5 (SEQ ID phage T4 member of NO: 8) enterobacteriaceae Table 2 below (which is separated in two parts) identifies the residues making up each domain in each Dda homologue (SEQ ID NOs: 8 to 23). .. Number of D/E Sequence vs. K/R Dda Homologue Identity to amino # (SEQ ID NO:) Habitat Uniprot Length 1993/% acids C Rma- Rhodothermus Mild D0MKQ2 678 21 −84/+85 2 DSM marinus halophile, (SEQ ID moderate NO: 9) thermophile >65 ° C. Csp Cyanothece sp. Marine B1X365 496 24 −76/+76 5 (SEQ ID (strain ATCC bacterium NO: 10) 51142) Sru Salinibacter Extremely Q25429 421 26 −78/+54 3 (SEQ ID ruber halophilic, NO: 11) 35-45 ° C. Sgo Sulfurimonas Habitat: B6BJ43 500 27 −72/+64 2 (SEQ ID gotlandica GD1 hydrotherma NO: 12) 1 vents, coastal sediments Vph12B8 Vibrio phage Host found M4MBC3 450 27 −62/+47 6 (SEQ ID henriette 12B8 in saltwater, NO: 13) stomach bug Vph Vibrio phage Host found I6XGX8 421 39 −55/+45 5 (SEQ ID phi-pp2 in saltwater, NO: 14) stomach bug Aph65 Aeromonas Host found E5DRP6 434 40 −57/+48 4 (SEQ ID phage 65 in NO: 15) fresh/brackish water, stomach bug AphCC2 Aeromonas Host found I6XH64 420 41 −53/+44 4 (SEQ ID phage CC2 in NO: 16) fresh/brackish water, stomach bug Cph Cronobacter Host K4FBD0 443 42 −59/+57 4 (SEQ ID phage vB CsaM member of NO: 17) GAP161 enterobacteriaceae Kph Klebsiella Host D5JF67 442 44 −59/+58 5 (SEQ ID phage KP15 member of NO: 18) enterobacteriaceae SphlME13 Stenotrophomonas Host found J7HXT5 438 51 −58/+59 7 (SEQ ID phage in soil NO: 19) IME13 AphAc42 Acinetobacter Host found E5EYE6 442 59 −53/+49 9 (SEQ ID phage Ac42 in soil NO: 20) SphSP18 Shigella phage Host E3SFA5 442 59 −55/+55 9 (SEQ ID SP18 member of NO: 21) enterobacteriaceae Yph Yersinia phage Host I7J3V8 439 64 −52/+52 7 (SEQ ID phiR1-RT member of NO: 22) enterobacteriaceae SphS16 Salmonella Host M1EA88 441 72 −56/+55 5 (SEQ ID phage S16 member of NO: 23) enterobacteriaceae 1993 Enterobateria Host P32270 439 100 −57/+58 5 (SEQ ID phage T4 member of NO: 8) enterobacteriaceae Table 2 below (which is separated in two parts) identifies the residues making up each domain in each Dda homologue (SEQ ID NOs: 8 to 23).
Article Title: Modified helicases
Article Snippet: .. Number Sequence of D/E Identity vs. K/R Dda Homologue to amino # (SEQ ID NO:) Habitat Uniprot Length 1993/% acids C Rma-DSM Rhodothermus Mild halophile, D0MKQ2 678 21 −84/+85 2 (SEQ ID marinus moderate NO: 9) thermophile >65° C. Csp Cyanothece sp. Marine B1X365 496 24 −76/+76 5 (SEQ ID (strain ATCC bacterium NO: 10) 51142) Sru Salinibacter ruber Extremely Q2S429 421 26 −78/+54 3 (SEQ ID halophilic, NO: 11) 35-45° C. Sgo Sulfurimonas Habitat: B6BJ43 500 27 −72/+64 2 (SEQ ID gotlandica GD1 hydrotherma NO: 12) 1 vents, coastal sediments Vph12B8 Vibrio phage Host found M4MBC3 450 27 −62/+47 6 (SEQ ID henriette 12B8 in saltwater, NO: 13) stomach bug Vph Vibrio phage Host found I6XGX8 421 39 −55/+45 5 (SEQ ID phi-pp2 in saltwater, NO: 14) stomach bug Aph65 Aeromonas Host found E5DRP6 434 40 −57/+48 4 (SEQ ID phage 65 in fresh/brackish NO: 15) water, stomach bug AphCC2 Aeromonas Host found I6XH64 420 41 −53/+44 4 (SEQ ID phage CC2 in fresh/brackish NO: 16) water, stomach bug Cph Cronobacter Host member of K4FBD0 443 42 −59/+57 4 (SEQ ID phage vB CsaM enterobacteri NO: 17) GAP161 aceae Kph Klebsiella phage Host member of D5JF67 442 44 −59/+58 5 (SEQ ID KP15 enterobacteri NO: 18) aceae SphlME13 Stenotrophomonas Host found in J7HXT5 438 51 −58/+59 7 (SEQ ID phage IME13 soil NO: 19) AphAc42 Acinetobacter Host found in E5EYE6 442 59 −53/+49 9 (SEQ ID phage Ac42 soil NO: 20) SphSP18 Shigella phage Host member of E3SFA5 442 59 −55/+55 9 (SEQ ID SP18 enterobacteri NO: 21) aceae Yph Yersinia phage Host member of I7J3V8 439 64 −52/+52 7 (SEQ ID phiR1-RT enterobacteri NO: 22) aceae SphS16 Salmonella Host member of M1EA88 441 72 −56/+55 5 (SEQ ID phage S16 enterobacteri NO: 23) aceae 1993 Enterobateria Host member of P32270 439 100 −57/+58 5 (SEQ ID phage T4 enterobacteri NO: 8) aceae Table 2 below (which is separated in two parts) identifies the residues making up each domain in each Dda homologue (SEQ ID NOs: 8 to 23). ..
Article Title: Modified enzymes
Article Snippet: .. TABLE 1 Number of D/E Sequence vs. K/R Dda Homologue Identity amino # (SEQ ID NO:) Habitat Uniprot Length to 1993/% acids C Rma- Rhodothermus Mild D0MKQ2 678 21 −84/+85 2 DSM marinus halophile, (SEQ ID moderate NO: 9) thermophile > 65° C. Csp Cyanothece sp. Marine B1X365 496 24 −76/+76 5 (SEQ ID (strain ATCC bacterium NO: 10) 51142) Sru Salinibacter Extremely Q2S429 421 26 −78/+54 3 (SEQ ID ruber halophilic, NO: 11) 35-45° C. Sgo Sulfurimonas Habitat: B6BJ43 500 27 −72/+64 2 (SEQ ID gotlandica hydrothermal NO: 12) GD1 vents, coastal sediments Vph12B8 Vibrio phage Host found M4MBC3 450 27 −62/+47 6 (SEQ ID henriette 12B8 in saltwater, NO: 13) stomach bug Vph Vibrio phage Host found I6XGX8 421 39 −55/+45 5 (SEQ ID phi-pp2 in saltwater, NO: 14) stomach bug Aph65 Aeromonas Host found in E5DRP6 434 40 −57/+48 4 (SEQ ID phage 65 fresh/brackish NO: 15) water, stomach bug AphCC2 Aeromonas Host found in I6XH64 420 41 −53/+44 4 (SEQ ID phage CC2 fresh/brackish NO: 16) water, stomach bug Cph Cronobacter Host member of K4FBD0 443 42 −59/+57 4 (SEQ ID phage vB CsaM enterobacteriaceae NO: 17) GAP161 Kph Klebsiella Host member of D5JF67 442 44 −59/+58 5 (SEQ ID phage KP15 enterobacteriaceae NO: 18) SphlME13 Stenotrophomonas Host found J7HXT5 438 51 −58/+59 7 (SEQ ID phage IME13 in soil NO: 19) AphAc42 Acinetobacter Host found E5EYE6 442 59 −53/+49 9 (SEQ ID phage Ac42 in soil NO: 20) SphSP18 Shigella phage Host member of E3SFA5 442 59 −55/+55 9 (SEQ ID SP18 enterobacteriaceae NO: 21) Yph Yersinia phage Host member of I7J3V8 439 64 −52/+52 7 (SEQ ID phiR1-RT enterobacteriaceae NO: 22) SphS16 Salmonella Host member of M1EA88 441 72 −56/+55 5 (SEQ ID phage S16 enterobacteriaceae NO: 23) 1993 Enterobateria Host member of P32270 439 100 −57/+58 5 (SEQ ID phage T4 enterobacteriaceae NO: 8) The helicase of the invention preferably comprises a variant of SEQ ID NO: 8 wherein the at least one amino acid which interacts with the sugar and/or base of one or more nucleotides in ssDNA is at least one of H82, N88, P89, F98, D121, V150, P152, F240, F276, S287, H396 and Y415. .. TABLE 1 Number of D/E Sequence vs. K/R Dda Homologue Identity amino # (SEQ ID NO:) Habitat Uniprot Length to 1993/% acids C Rma- Rhodothermus Mild D0MKQ2 678 21 −84/+85 2 DSM marinus halophile, (SEQ ID moderate NO: 9) thermophile > 65° C. Csp Cyanothece sp. Marine B1X365 496 24 −76/+76 5 (SEQ ID (strain ATCC bacterium NO: 10) 51142) Sru Salinibacter Extremely Q2S429 421 26 −78/+54 3 (SEQ ID ruber halophilic, NO: 11) 35-45° C. Sgo Sulfurimonas Habitat: B6BJ43 500 27 −72/+64 2 (SEQ ID gotlandica hydrothermal NO: 12) GD1 vents, coastal sediments Vph12B8 Vibrio phage Host found M4MBC3 450 27 −62/+47 6 (SEQ ID henriette 12B8 in saltwater, NO: 13) stomach bug Vph Vibrio phage Host found I6XGX8 421 39 −55/+45 5 (SEQ ID phi-pp2 in saltwater, NO: 14) stomach bug Aph65 Aeromonas Host found in E5DRP6 434 40 −57/+48 4 (SEQ ID phage 65 fresh/brackish NO: 15) water, stomach bug AphCC2 Aeromonas Host found in I6XH64 420 41 −53/+44 4 (SEQ ID phage CC2 fresh/brackish NO: 16) water, stomach bug Cph Cronobacter Host member of K4FBD0 443 42 −59/+57 4 (SEQ ID phage vB CsaM enterobacteriaceae NO: 17) GAP161 Kph Klebsiella Host member of D5JF67 442 44 −59/+58 5 (SEQ ID phage KP15 enterobacteriaceae NO: 18) SphlME13 Stenotrophomonas Host found J7HXT5 438 51 −58/+59 7 (SEQ ID phage IME13 in soil NO: 19) AphAc42 Acinetobacter Host found E5EYE6 442 59 −53/+49 9 (SEQ ID phage Ac42 in soil NO: 20) SphSP18 Shigella phage Host member of E3SFA5 442 59 −55/+55 9 (SEQ ID SP18 enterobacteriaceae NO: 21) Yph Yersinia phage Host member of I7J3V8 439 64 −52/+52 7 (SEQ ID phiR1-RT enterobacteriaceae NO: 22) SphS16 Salmonella Host member of M1EA88 441 72 −56/+55 5 (SEQ ID phage S16 enterobacteriaceae NO: 23) 1993 Enterobateria Host member of P32270 439 100 −57/+58 5 (SEQ ID phage T4 enterobacteriaceae NO: 8) The helicase of the invention preferably comprises a variant of SEQ ID NO: 8 wherein the at least one amino acid which interacts with the sugar and/or base of one or more nucleotides in ssDNA is at least one of H82, N88, P89, F98, D121, V150, P152, F240, F276, S287, H396 and Y415.
Article Title: Modified enzymes
Article Snippet: .. TABLE 1 Number Sequence of D/E Identity vs. K/R Dda Homologue to 1993/ amino (SEQ ID NO:) Habitat Uniprot Length % acids # C Rma- Rhodothermus Mild D0MKQ2 678 21 −84/+85 2 DSM marinus halophile, (SEQ ID moderate NO: 9) thermophile >65° C. Csp Cyanothece sp. Marine B1X365 496 24 −76/+76 5 (SEQ ID (strain ATCC bacterium NO: 10) 51142) Sru Salinibacter Extremely Q2S429 421 26 −78/+54 3 (SEQ ID ruber halophilic, NO: 11) 35-45° C. Sgo Sulfurimonas Habitat: B6BJ43 500 27 −72/+64 2 (SEQ ID gotlandica GD1 hydrothermal NO: 12) vents, coastal sediments Vph12B8 Vibrio phage Host found M4MBC3 450 27 −62 /+47 6 (SEQ ID henriette 12B8 in saltwater, NO: 13) stomach bug Vph Vibrio phage Host found I6XGX8 421 39 −55/+45 5 (SEQ ID phi-pp2 in saltwater, NO: 14) stomach bug Aph65 Aeromonas Host found E5DRP6 434 40 −57/+48 4 (SEQ ID phage 65 in NO: 15) fresh/brackish water, stomach bug AphCC2 Aeromonas Host found I6XH64 420 41 −53/+44 4 (SEQ ID phage CC2 in NO: 16) fresh/brackish water, stomach bug Cph Cronobacter Host K4FBD0 443 42 −59/+57 4 (SEQ ID phage vB CsaM member of NO: 17) GAP161 enterobacteriaceae Kph Klebsiella Host D5JF67 442 44 −59/+58 5 (SEQ ID phage KP15 member of NO: 18) enterobacteriaceae SphlME13 Stenotrophomonas Host found J7HXT5 438 51 −58/+59 7 (SEQ ID phage in soil NO: 19) IME13 AphAc42 Acinetobacter Host found E5EYE6 442 59 −53/+49 9 (SEQ ID phage Ac42 in soil NO: 20) SphSP18 Shigella phage Host E3SFA5 442 59 −55/+55 9 (SEQ ID SP18 member of NO: 21) enterobacteriaceae Yph Yersinia phage Host I7J3V8 439 64 −52/+52 7 (SEQ ID phiR1-RT member of NO: 22) enterobacteriaceae SphS16 Salmonella Host M1EA88 441 72 −56/+55 5 (SEQ ID phage S16 member of NO: 23) enterobacteriaceae 1993 Enterobateria Host P32270 439 100 −57/+58 5 (SEQ ID phage T4 member of NO: 8) enterobacteriaceae The helicase of the invention preferably comprises a variant of SEQ ID NO: 8 wherein the at least one amino acid which interacts with the sugar and/or base of one or more nucleotides in ssDNA is at least one of H82, N88, P89, F98, D121, V150, P152, F240, F276, S287, H396 and Y415. ..
Variant Assay:Article Title: Modified enzymes
Article Snippet: .. TABLE 1 Number of D/E Sequence vs. K/R Dda Homologue Identity amino # (SEQ ID NO:) Habitat Uniprot Length to 1993/% acids C Rma- Rhodothermus Mild D0MKQ2 678 21 −84/+85 2 DSM marinus halophile, (SEQ ID moderate NO: 9) thermophile > 65° C. Csp Cyanothece sp. Marine B1X365 496 24 −76/+76 5 (SEQ ID (strain ATCC bacterium NO: 10) 51142) Sru Salinibacter Extremely Q2S429 421 26 −78/+54 3 (SEQ ID ruber halophilic, NO: 11) 35-45° C. Sgo Sulfurimonas Habitat: B6BJ43 500 27 −72/+64 2 (SEQ ID gotlandica hydrothermal NO: 12) GD1 vents, coastal sediments Vph12B8 Vibrio phage Host found M4MBC3 450 27 −62/+47 6 (SEQ ID henriette 12B8 in saltwater, NO: 13) stomach bug Vph Vibrio phage Host found I6XGX8 421 39 −55/+45 5 (SEQ ID phi-pp2 in saltwater, NO: 14) stomach bug Aph65 Aeromonas Host found in E5DRP6 434 40 −57/+48 4 (SEQ ID phage 65 fresh/brackish NO: 15) water, stomach bug AphCC2 Aeromonas Host found in I6XH64 420 41 −53/+44 4 (SEQ ID phage CC2 fresh/brackish NO: 16) water, stomach bug Cph Cronobacter Host member of K4FBD0 443 42 −59/+57 4 (SEQ ID phage vB CsaM enterobacteriaceae NO: 17) GAP161 Kph Klebsiella Host member of D5JF67 442 44 −59/+58 5 (SEQ ID phage KP15 enterobacteriaceae NO: 18) SphlME13 Stenotrophomonas Host found J7HXT5 438 51 −58/+59 7 (SEQ ID phage IME13 in soil NO: 19) AphAc42 Acinetobacter Host found E5EYE6 442 59 −53/+49 9 (SEQ ID phage Ac42 in soil NO: 20) SphSP18 Shigella phage Host member of E3SFA5 442 59 −55/+55 9 (SEQ ID SP18 enterobacteriaceae NO: 21) Yph Yersinia phage Host member of I7J3V8 439 64 −52/+52 7 (SEQ ID phiR1-RT enterobacteriaceae NO: 22) SphS16 Salmonella Host member of M1EA88 441 72 −56/+55 5 (SEQ ID phage S16 enterobacteriaceae NO: 23) 1993 Enterobateria Host member of P32270 439 100 −57/+58 5 (SEQ ID phage T4 enterobacteriaceae NO: 8) The helicase of the invention preferably comprises a variant of SEQ ID NO: 8 wherein the at least one amino acid which interacts with the sugar and/or base of one or more nucleotides in ssDNA is at least one of H82, N88, P89, F98, D121, V150, P152, F240, F276, S287, H396 and Y415. .. TABLE 1 Number of D/E Sequence vs. K/R Dda Homologue Identity amino # (SEQ ID NO:) Habitat Uniprot Length to 1993/% acids C Rma- Rhodothermus Mild D0MKQ2 678 21 −84/+85 2 DSM marinus halophile, (SEQ ID moderate NO: 9) thermophile > 65° C. Csp Cyanothece sp. Marine B1X365 496 24 −76/+76 5 (SEQ ID (strain ATCC bacterium NO: 10) 51142) Sru Salinibacter Extremely Q2S429 421 26 −78/+54 3 (SEQ ID ruber halophilic, NO: 11) 35-45° C. Sgo Sulfurimonas Habitat: B6BJ43 500 27 −72/+64 2 (SEQ ID gotlandica hydrothermal NO: 12) GD1 vents, coastal sediments Vph12B8 Vibrio phage Host found M4MBC3 450 27 −62/+47 6 (SEQ ID henriette 12B8 in saltwater, NO: 13) stomach bug Vph Vibrio phage Host found I6XGX8 421 39 −55/+45 5 (SEQ ID phi-pp2 in saltwater, NO: 14) stomach bug Aph65 Aeromonas Host found in E5DRP6 434 40 −57/+48 4 (SEQ ID phage 65 fresh/brackish NO: 15) water, stomach bug AphCC2 Aeromonas Host found in I6XH64 420 41 −53/+44 4 (SEQ ID phage CC2 fresh/brackish NO: 16) water, stomach bug Cph Cronobacter Host member of K4FBD0 443 42 −59/+57 4 (SEQ ID phage vB CsaM enterobacteriaceae NO: 17) GAP161 Kph Klebsiella Host member of D5JF67 442 44 −59/+58 5 (SEQ ID phage KP15 enterobacteriaceae NO: 18) SphlME13 Stenotrophomonas Host found J7HXT5 438 51 −58/+59 7 (SEQ ID phage IME13 in soil NO: 19) AphAc42 Acinetobacter Host found E5EYE6 442 59 −53/+49 9 (SEQ ID phage Ac42 in soil NO: 20) SphSP18 Shigella phage Host member of E3SFA5 442 59 −55/+55 9 (SEQ ID SP18 enterobacteriaceae NO: 21) Yph Yersinia phage Host member of I7J3V8 439 64 −52/+52 7 (SEQ ID phiR1-RT enterobacteriaceae NO: 22) SphS16 Salmonella Host member of M1EA88 441 72 −56/+55 5 (SEQ ID phage S16 enterobacteriaceae NO: 23) 1993 Enterobateria Host member of P32270 439 100 −57/+58 5 (SEQ ID phage T4 enterobacteriaceae NO: 8) The helicase of the invention preferably comprises a variant of SEQ ID NO: 8 wherein the at least one amino acid which interacts with the sugar and/or base of one or more nucleotides in ssDNA is at least one of H82, N88, P89, F98, D121, V150, P152, F240, F276, S287, H396 and Y415.
Article Title: Modified enzymes
Article Snippet: .. TABLE 1 Number Sequence of D/E Identity vs. K/R Dda Homologue to 1993/ amino (SEQ ID NO:) Habitat Uniprot Length % acids # C Rma- Rhodothermus Mild D0MKQ2 678 21 −84/+85 2 DSM marinus halophile, (SEQ ID moderate NO: 9) thermophile >65° C. Csp Cyanothece sp. Marine B1X365 496 24 −76/+76 5 (SEQ ID (strain ATCC bacterium NO: 10) 51142) Sru Salinibacter Extremely Q2S429 421 26 −78/+54 3 (SEQ ID ruber halophilic, NO: 11) 35-45° C. Sgo Sulfurimonas Habitat: B6BJ43 500 27 −72/+64 2 (SEQ ID gotlandica GD1 hydrothermal NO: 12) vents, coastal sediments Vph12B8 Vibrio phage Host found M4MBC3 450 27 −62 /+47 6 (SEQ ID henriette 12B8 in saltwater, NO: 13) stomach bug Vph Vibrio phage Host found I6XGX8 421 39 −55/+45 5 (SEQ ID phi-pp2 in saltwater, NO: 14) stomach bug Aph65 Aeromonas Host found E5DRP6 434 40 −57/+48 4 (SEQ ID phage 65 in NO: 15) fresh/brackish water, stomach bug AphCC2 Aeromonas Host found I6XH64 420 41 −53/+44 4 (SEQ ID phage CC2 in NO: 16) fresh/brackish water, stomach bug Cph Cronobacter Host K4FBD0 443 42 −59/+57 4 (SEQ ID phage vB CsaM member of NO: 17) GAP161 enterobacteriaceae Kph Klebsiella Host D5JF67 442 44 −59/+58 5 (SEQ ID phage KP15 member of NO: 18) enterobacteriaceae SphlME13 Stenotrophomonas Host found J7HXT5 438 51 −58/+59 7 (SEQ ID phage in soil NO: 19) IME13 AphAc42 Acinetobacter Host found E5EYE6 442 59 −53/+49 9 (SEQ ID phage Ac42 in soil NO: 20) SphSP18 Shigella phage Host E3SFA5 442 59 −55/+55 9 (SEQ ID SP18 member of NO: 21) enterobacteriaceae Yph Yersinia phage Host I7J3V8 439 64 −52/+52 7 (SEQ ID phiR1-RT member of NO: 22) enterobacteriaceae SphS16 Salmonella Host M1EA88 441 72 −56/+55 5 (SEQ ID phage S16 member of NO: 23) enterobacteriaceae 1993 Enterobateria Host P32270 439 100 −57/+58 5 (SEQ ID phage T4 member of NO: 8) enterobacteriaceae The helicase of the invention preferably comprises a variant of SEQ ID NO: 8 wherein the at least one amino acid which interacts with the sugar and/or base of one or more nucleotides in ssDNA is at least one of H82, N88, P89, F98, D121, V150, P152, F240, F276, S287, H396 and Y415. ..
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